Denim sales, which bounced back two years ago, are looking good with robust growth. However, Chatterjee steps into the top slot at a time when the parent company has put Dockers, a significant revenue earner and a brand that played a crucial role in the company’s fortunes in India, on sale. Chatterjee says he will expand merchandise and keep his focus on the premium end.
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Measurement of surface 2 subunit expression using flow cytometryMeasurement of surface expression of GABAA receptor 2 subunits using flow cytometry has been described previously.8 ,9 Briefly, HEK293T cells were transfected using polyethylenimine reagent (40 kD, Polysciences) at a DNA:transfection reagent ratio of 1:2.5 and harvested 48hours after transfection. To express wild type (122s) and mutant (12(T287P)2s) receptors, a total of 3g of subunit cDNAs were transfected at a ratio of 1:1:1 into 6cm dishes for most experiments except for whole cell recording. For mock or single subunit expression, empty pcDNA3.1 vector was added to make a final cDNA transfection amount to 3g.
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Strainers with large holes (like pasta strainers) don’t work well the smaller grains may pass right through into your kefir drink, rendering it gritty andlumpy(and unstorable it will continue to ferment quickly in the fridge). A sterile wood or plastic spoon is also handy to help strain the kefir. You may want to have some yummy freshfruit or othervanilla extract on hand to flavor your kefir, though it isyummy plain, too!I am just learning about kefir, as I am trying to become more health conscious, and trying to add more healthy, nutritional food to my diet.